Biosynthesis of levan and a new method for the assay of levansucrase activity.
نویسنده
چکیده
The polysaccharide levan was synthesized in a solidified agar medium containing sucrose as a source of fructose. The biosynthesis was achieved by the enzyme levansucrase (2,6-fructan-d-glucose 6-fructosyltransferase, EC 2.4.1.10), a small quantity of which was placed in circular wells cut in the agar gel. The enzyme slowly diffused through the agar-sucrose medium and the synthesis of levan was observed as circular white areas, the size of which was dependent on the time of incubation and the concentration of enzyme used.
منابع مشابه
Box-Wilson Design for Optimization of in vitro Levan Production and Levan Application as Antioxidant and Antibacterial Agents
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ورودعنوان ژورنال:
- The Biochemical journal
دوره 125 1 شماره
صفحات -
تاریخ انتشار 1971